Methocarbamol and/or atropine sulfate antagonism study of Flucythrinate was carried out in male mice of SIc:ICR strain at 6 weeks of age.
145㎎/㎏ was set as the LD_(50), value of Flucythrinate in the control and therapeutic groups. The mortality rate of the control group was 60% and showed CS syndrome; head raising, salivation, abnormal gait and rolling and jumping(Choreoathetosis).
Methocarbamol was initially injected intraperitoneally at a dose of 350 ㎎/㎏(body weight) when salivation caused by Flucythrinate was observed, followed by repeated doses of 1a0 ㎎/㎏ whenever rolling and jumping was observed. The mortality rate (30%) was markedly decreased from the control (60%), and survival times of dead animals were prolonged.
Atropine sulfate was injected subcutaneously at a dose of 25 ㎎/㎏ when salivation caused by Flucythrinate was observed. The results was shown adversely; the mortality rate was increased 60% to 100% and survival times of the dead animals were shortened.
Methocarbamol and atropine sulfate were simultaneously injected as the same methods of Methocarbamol and atropine sulfate injection. The mortality rate was slightly decreased and survival time of dead animals were also slightly prolonged.
Methocarbamol and atropine sulfate was effective in depressing clinical signs such as salivation, abnormal gait and choreoathetosis and still effective in the improvement of the mortality rate produced by Flucythrinate, however, atropine sulfate was not therapeutically effective in mortality.
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